Detection of some virulence factors of Pseudomonas aeruginosa isolated from burns and wounds

Muthanna Medical Journal
Volume 9, Issue 1,  2022 Page 15-28
http://dx.doi.org/10.52113/1/1/2022-15-28

Shaimaa MS. Zainulabdeen ¹, Khawlah Jebur Khalaf ², Jehan Abdul Sattar Salman ²

Correspondence author: Shimaa.majeed@mu.edu.iq
¹ Department of Microbiology, College of Medicine, Al-Muthanna university
² Department of Biology, College of Science, Mustansiriyah University  
Received 09 March 2022, Accepted 19 May 2022, Available online 20 May 2022
Copyright © 2022 SM . This is article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Pseudomonas aeruginosa is a pathogenic microbe that has a wide variety of opportunistic infections, including surgical site, urinary tract, pneumonia and bloodstream, wounds and burns, ear, nose, and throat infections, as nosocomial infections in the hospital intensive care units (ICUs). Nineteen isolates of P. aeruginosa from burns and wounds were collected. All isolates also were subjected to the cultural, biochemical tests as well as vitek 2 system. Moreover, these isolates had been tested for susceptibility to (10) antibiotic discs and also detection of some virulence factors included hemolysin, pyocyanin, swarming motility, and biofilm formation. Most isolates were resistant to tetracycline, imipenem, and doxycycline, whereas they showed more sensitive to amikacin, tobramycin, and aztreonam. P. aeruginosa had been showed different range for hemolysin activity, pyocyanin and swarming motility, furthermore they varied (moderate and weak) for biofilm formation.

Keywords: Pseudomonas aeruginosa, Virulence factors, Wounds and burns