Forkhead Box O1 genetic variants and predisposition to gestational diabetes mellitus in Sudanese women

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Muthanna Medical Journal

Volume 10, Issue 2,  2023 Page 160-172
http://dx.doi.org/10.52113/1/1/2023-160-172

Mashair. E. Ezeldein ¹*,  Hani. Y. Zaki 1, Elhassan M. Esihag ², Adil Mergani ³, Mona Ahmed Eltyeb ¹, Badreldin Elsonni Abdalla ¹

Correspondence author: mashairezeldein@gmail.com
¹ Department of Biochemistry and Nutrition, Faculty of Medicine, University of Gezira, Sudan.
Department of Obstetrics and Gynecology, Faculty of Medicine University of Gezira, Sudan.
Department of Molecular Biology, National Cancer Institute University of Gezira, Sudan.
Received 09 May 2023; revised 11 June 2023; accepted 23 July 2023, available online 11 August 2023.
Copyright © 2023 Ezeldein, et al. This is article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Abstract

Forkhead box class O1 (FOXO1) is intimately linked.to Gestational Diabetes Mellitus (GDM). However, the association between genetic variations and susceptibility to GDM has not been explored. We aimed to detect the association of the common polymorphisms of FOXO1 gene and predisposition to GDM. In this study, a total of 193 pregnant Sudanese women aged (18-39 years) were classified into two main groups: 94 Normal pregnant women and 99 pregnant women diagnosed with GDM. The genotypes of two selected SNPs rs17446614 and rs2701858 in FOXO1 gene were determined by PCR-CTPP with proper primer designing and PCR conditions. Insulin homeostasis model assessment of insulin resistance (HOMA IR) and lipid profile parameters were also examined. We observed that differences in genotype frequencies of rs17446614, rs2701858 in FOXO1 for GDM vs normally pregnant women (NP) were statistically significant, particularly in the dominant model considering the major genotypes as a reference. Furthermore, we detected common haplotypes, A rs17446614 C rs2701858 (OR=1.89, 95%CI: 1.11-3.20) and G rs17446614 T rs2701858 (OR=2.13, 95%CI: 1.14 -3.95) within FOXO1gene showed significant difference. There was no association between genotype and biochemical parameters related to GDM.  In conclusion, genetic variant rs17446614 and variant rs2701858 in FOXO1 gene may contribute to the risk of GDM in Sudanese women.

Keywords: Gestational Diabetes Mellitus, FOXO1, Sudanese Women