Preparation of wound dressings using levan from Lactobacillus gasseri and polyvinyl alcohol polymer

Posted on by Muthanna Medical Journal

Research article

Volume 11, Issue 2,  2024.  Page 104-123    10.52113/1/1/2024-2-103

Authors
Jehan Abdul Sattar Salman *1 , Shaimaa MS Zainulabdeen 2, Khawlah Jebur Khalaf 1    

*Correspondence author: Dr.jehan@uomustansiriyah.edu.iq

*1 Department of Biology, College of Science, Mustansiriyah University, Baghdad, Iraq.
2 Department of MicroBiology, College of Medicine, Al-Muthanna University, Al-Muthanna, Iraq
 Received 17 July 2024; revised 22 August 2024; accepted 19 September 2024, available online 15 October 2024.
Copyright © 2024 Salman, et al. This is article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited

Abstract

Levan had been extracted, purified, and characterized from Lactobacillus gasseri. Levan characterization had been carried out using "thin-layer chromatography (TLC)", 1HNuclear-MagneticsResonances (HNMR), and Fouriere-transforms infrared spectroscopies (FTIR). Characterization demonstrated that pure levan is a homopolysaccharide with just fructose and atypical for carbohydrate bond area of 868 cm-1. The maximum water solubility index (WSI) percentage was 90% at a conc. of 100 mg/5 ml, while the highest water holding capacity value had been 252 at a conc. of 50 mg/5 ml. The wound dressings were prepared using purified levan from L.gasseri and its blend with polyvinyl alcohol (PVA) polymer, and the polymer add-on percentage and the reduction of Pseudomonas aeruginosa growth were calculated. The proportion of polymer applied to cotton gauze has been shown that the polymer add-on for three types of polymers include levan, PVA, and the mixture of levan and PVA were (177.00, 81.50, and 290.50) % respectively. The reduction of P. aeruginosa growth by the polymers-coated gauze investigated that there was no bacterial growth in levan-PVA blend coated cotton gauze and the bacterial reduction percentage was 100% compared to levan coated cotton gauze with 52.00% for burn isolate and 66.66% for wound isolate, while the bacterial reduction of PVA coated cotton gauze was 43.00% for burn isolate and 55.55% for wound isolate. On the other hand, the present study included evaluating the effectiveness of the prepared levan-PVA cotton gauze bandage through its use in dressing rabbits that were injured and infected with P.aeruginosa. The present results showed that the prepared levan-PVA cotton gauze bandage led to faster wound healing, without harmful effects, and enhanced wound healing during the first week compared to traditional cotton gauze alone and antibiotic cotton gauze.

Keywords: Levan, PVA, Anti-bacterial, Wound dressing

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Volume 10, Issue 2, 2023

 

 

 

 

 

Volume 11, Issue 2, 2024

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Salman JA, Zainulabdeen S, Khalaf KJ. Preparation of wound dressings using levan from Lactobacillus gasseri and polyvinyl alcohol polymer. Muthanna Medical Journal. 2024; 11(2):104-123.