Muthanna Medical Journal
Volume 4, Issue 2, 2017 Page 179-188
http://dx.doi.org/10.18081/2410-4590/2017-179-190
Suaad Abid Fazaa Al-Miyah 1
* Correspondence author: Drmicro2013@gmail.com
1Medical mathematics Depart., College of computer science and information technology, Qadisiya University
Received 11 July 2017, Accepted 20 September 2017, Available online 27 October 2017
Abstract
Food borne pathogens are major impediment of the public health and economic development, mostly Enterobacteriaceae is inherent foodborne pathogens of which E. herbicola have recently emerged significant in ready eat food. The aim of this study was to determine quantitatively of viable bacterial isolates and molecular diagnosis of Erwinia herbicola by SYBR Green real-time PCR in nuts. The bacterial species isolates of nuts are Coagulase + Staphylococcus, E. coli, Enterobacter cloacae and Erwinia herbicola. The highest rate of Coagulase + Staphylococcus species of two hazelnuts and two cashew samples and Erwinia herbicola of one almond sample, theses bacterial isolates from nuts were present between microbiological unsatisfactory and marginal levels, however in present study selected E. herbicola to molecular identification by used SYBR Green qPCR assay to amplify the rep gene. The specificity of assay was represented molecular quantitation correlation was between ct values and enumeration on aerobic plate agar count of bacteria in nuts, the results showed that assay is specific diagnosis of E. herbicola through ct values ranging from 20.5.to 23.6 for four samples.
Keywords: Enterobacteriaceae; SYBR Green qPCR; Staphylococcus