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Article Type

Article

Abstract

Polymicrobial biofilms, composed of fungi and bacteria such as Candida and Staphylococcus aureus, exhibit interactions dependent on environmental factors and organism type. The objective of this investigation was to evaluate the ability of S. aureus and Candida species to construct mono and polymicrobial biofilms. Candida species and S. aureus were isolated from various clinical sources. The identification of these isolates was based on morphological and biochemical characteristics as well as using Vitek 2 compact. Biofilm formation was evaluated using flat-bottom microtiter plate assay with crystal violet. A total of 41 S. aureus, 15 C. tropicalis, 14 C. kruesi and 7 C. albicans were tested to investigate their ability in biofilm formation. The results indicated that all the isolates of both S. aureus and Candida species formed biofilms. Based on biofilm categories, C. tropicalis and C. kruesi formed biofilms stronger than C. albicans and S. aureus. However, polymicrobial biofilms results indicated that the presence of Candida species with S. aureus increased the biofilm formation via synergetic action. In conclusion, confirmed the ability of Candida and S. aureus construct mono and polymicrobial biofilms.

Keywords

Polymicrobial, Biofilm, S. aureus, Candida spp

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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