Purified β-Glucan from Enterococcus faecium as Antibacterial and Antibiofilm Agent Against MDR Gram-Positive Wound Infections Causative Bacteria

Authors

Entissar Hussain Abdulhameed, Department of Biology, College of Science, Mustansiriyah University, Baghdad, IraqFollow
Jehan Abdul Sattar Salman, Department of Biology, College of Science, Mustansiriyah University, Baghdad, IraqFollow

Article Type

Article

Abstract

Background: The investigation focused on MDR- gram positive isolated from wounds infections and assessed their ability for biofilm formation. The effect of purified β- glucan produced from E. faecium on the growth and biofilm of MDR wound isolates was studied. Methods: One hundred and thirty-five samples were collected from hospitals in Baghdad, Iraq, resulting in the isolation of nine isolates of Staphylococcus spp. and Streptococcus spp., which were grown, examined microscopically, biochemically, and identified by Vitek2. Their susceptibility to ten antibiotics were evaluated, and their capacity to produce biofilms were examined. The antibiofilm impact of pure β-glucan was evaluated after 24 hours. Results: Eight from nine isolates were MDR and the ratio of resistance to Carbenicillin was 100% for all isolates and most of them produced biofilm in different proportion. The β-Glucan was purified from the Enterococcus faecium and its antibacterial efficacy was assessed by minimum inhibitory concentration (MIC) with concentration ranging between 9.37 to 150mg/ml, the MIC for those was 75 and 150 mg/ml. The maximum inhibition percentage for creating biofilm was 90% against MDR S. aureus ₍₅₎, whereas the minimum inhibition percentage obtained was 20% against S. aureus _(4). The FE-SEM image illustrating the antibiofilm effect of β-glucan against S. aureus ₍₅₎ clearly revealed the inhibitory effect of pure β-glucan on the biofilm formation of S. aureus _(5). Conclusion: Enterococcus faecium possesses the capability to synthesize β-glucan. Purified β-glucan exhibited antibacterial and antibiofilm properties against multidrug-resistant gram-positive bacteria that contribute to wound infections.

Keywords

MDR gram positive isolates, Inhibition of biofilm formation, β-glucan, E. faecium

Recommended Citation

Abdulhameed, Entissar Hussain and Salman, Jehan Abdul Sattar (2025) "Purified β-Glucan from Enterococcus faecium as Antibacterial and Antibiofilm Agent Against MDR Gram-Positive Wound Infections Causative Bacteria," Muthanna Medical Journal: Vol. 12: Iss. 3, Article 9.
Available at: https://muthmj.mu.edu.iq/journal/vol12/iss3/9

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This work is licensed under a Creative Commons Attribution 4.0 International License.