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Article Type

Article

Abstract

Short tandem repeats (STRs) are highly polymorphic forensic DNA markers widely used in population genetics, paternity testing, forensic genetics, and human identification. In this research we aimed to provide a comprehensive evaluation of STR alleles as forensic markers and study genetic diversity across five major regions in Iraqis. Collection of buccal swab samples from 1345 random unrelated Iraqis from different cities representing the north, west, east, south, and center of Iraq were chosen for analysis of population and forensic parameters, involving DNA extraction, PCR for amplification, and capillary electrophoresis by using a genetic analyzer. In addition, study the genetic diversity distance using the STAF2.2.2 software program between Iraqi major regions. The results indicated the AmpFiSTR® Identifier™  kit is suitable for genetic and forensic applications in our country's population. The south cities and the center are genetically closer than the other cities. This study displays the suitability and efficiency of STR alleles biomarkers as tools for DNA profiling analysis and forensic case investigations as well as their impact in assessing genetic distances among major regions of Iraqi genetic populations

Keywords

STRs, Genetic diversity, Iraq population, STRAF analysis

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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