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Article Type

Article

Abstract

Background: Amino-sulfonic derivatives are recognized for their diverse biological roles, particularly in modulating physiological environments and cellular protection. Understanding their interaction with enzyme systems like carbonic anhydrase (CA) is crucial for developing therapeutic strategies against oxidative stress and metabolic imbalances.
Objective: This study aims to systematically evaluate the antioxidant, cytoprotective, and pH-modulating activities of four amino-sulfonic derivatives—taurine (TA), pyridine-3-sulfonic acid (P-3-S), sulfamic acid (SA), and hydroxylamine-O-sulfonic acid (HASA)—using CA-containing systems derived from healthy and leukemia samples.
Materials and methods: Antioxidant capacity was determined through DPPH radical and H2O2 scavenging assays. Cytoprotection was assessed via lactate dehydrogenase (LDH) release and anti-hemolytic activity in erythrocyte models. Additionally, time- and concentration-dependent experiments were conducted to monitor pH elevation in CA-containing systems from both healthy and leukemia sources.
Results and conclusion: Pyridine-3-sulfonic acid demonstrated the strongest effects, reaching 91.05% DPPH and 90.01% H2O2 scavenging at 300 μg/mL, with significantly reduced LDH leakage and hemolysis. Optimal pH modulation was observed at 30–40 min across all samples. The results conclude that these derivatives exhibit structure-dependent bioactivity and effectively regulate CA-associated proton balance, highlighting their potential as protective agents in acidic pathological environments.

Keywords

Amino sulfonic acids, Antioxidant, Carbonic anhydrase, Anti-hemolytic test

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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